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qPCR is cost-productive and accessible given that the laboratory settings, machinery and reagents are just like These essential for making ready the NGS libraries.

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We quantified total microbes employing common bacterial primers [29] by qPCR in 114 Grownup fecal DNA samples which were analyzed for microbiota composition applying read more Illumina MiSeq for 16S rRNA gene amplicon sequencing [14]. The qPCR threshold cycle (Ct) values were being transformed into the estimates of bacterial genomes existing in 1 g of feces being a proxy of complete bacterial counts.

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